Concordant Segregation of the Expression of Sv40 T Antigen and Human Chromosome 7 in Mouse-human Hybrid Subclones
نویسندگان
چکیده
Subcloning of Simian virus 40 (SV40) T antigen-positive mouse-human hybrids, derived from the fusion of mouse cells deficient in thymidine kinase with SV40-transformed Lesch Nyhan fibroblasts, resulted in their segregation into T antigen-positive and negative subclones. Positive correlation between the presence of human chromosome 7 and the expression of SV40 T antigen was established in the subclones examined. These results negate the possibility of a transfer of the SV40 genome to a mouse chromosome.
منابع مشابه
Assignment of the T-antigen gene of simian virus 40 to human chromosome C-7.
Hybrid cell clones between mouse cells deficient in thymidine kinase (EC 2.7.1.21) and two different human cell lines transformed by simian virus 40 (SV40) and deficient in hypoxanthine phosphoribosyltransferase (EC 2.4.2.8) were examined for SV40 tumor (T) antigen(s). Concordant segregation of the gene(s) for SV40 T antigen and human chromosome C-7 was observed in these hybrids. The human chro...
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عنوان ژورنال:
- The Journal of Experimental Medicine
دوره 139 شماره
صفحات -
تاریخ انتشار 1974